A spectrophotometer is a measuring instrument that samples a reflective object or a transmissive object with a discontinuous wavelength. Because of the different structures of molecules of different objects, the absorption capacity of light of different wavelengths is also different. Therefore, each object has a specific absorption spectrum. An instrument that can separate each monochromatic light from a mixture of various wavelengths and measure its intensity is called a spectrophotometer.
Spectrophotometry is the development of colorimetry. The colorimetric method is limited to the visible light region, and the spectrophotometric law can be extended to the ultraviolet region and the infrared region. The spectrophotometric rule requires near-true monochromatic light with a spectral bandwidth of no more than 3-5 nm and a wavelength of less than 1 nm in the ultraviolet region, with high precision from prisms or gratings. Spectrophotometer? It is an instrument for quantitative and qualitative analysis of substances by spectrophotometry. The spectrophotometer can be divided into an ultraviolet spectrophotometer, a visible spectrophotometer (or a colorimeter), an infrared spectrophotometer or an atomic absorption spectrophotometer.
Spectrophotometers have become routine instruments in modern molecular biology laboratories. Often used for nucleic acid, protein quantification and quantification of bacterial growth concentrations. The instrument consists mainly of a light source, a monochromator, a sample chamber, a detector, a signal processor, and a display and storage system.
Spectrophotometer featuresUnique dual-optical, dual-beam optical system with higher resolution, lower stray light, better stability and reliability, and more accurate analysis;
Adopting 320*240 dot matrix highlighting 6†LCD display, the display is clear and the information is complete;
The unique long-path optical path design makes the instrument resolution higher, especially suitable for the powerful data processing function of micro-test, so that the test results can be fully applied, and the user's editing is simpler and faster;
Suspension optical system design, the overall optical path is independently fixed on the 16mm thick aluminum deformation-free base, the deformation of the bottom plate and the external vibration have no effect on the optical system, thus greatly improving the stability and reliability of the instrument. ;
Adopt synchronous sinusoidal mechanism, high wavelength accuracy and good repeatability;
Using an ARM system;
The 0.1/0.2/0.5/1.0/2.0/4.0 six-speed spectral bandwidth is automatically selected to meet the measurement needs of different users;
24-bit high-speed, high-precision A/D conversion, higher instrument accuracy and faster response;
The main components are imported, which makes the instrument less stray light, stable and reliable.
More powerful, the host can independently perform photometric measurement, quantitative measurement, spectral scanning, dynamics, DNA/protein testing, multi-wavelength testing and data printing;
Taking full account of the usage habits of different users, this series of instruments are equipped with the spectrum analysis software of Yuanfang Company. When online operation, in addition to all the test functions of the host, it can realize more powerful data processing functions and make data storage. Reach unlimited.
The spectrophotometer uses a light source that can generate multiple wavelengths, and passes through a series of spectroscopic devices to generate a light source of a specific wavelength. After the light passes through the sample to be tested, part of the light is absorbed, and the absorbance of the sample is calculated, thereby converting into a sample concentration. . The absorbance of the sample is proportional to the concentration of the sample.
When monochromatic light radiation passes through the solution of the substance to be tested, the amount absorbed by the substance is proportional to the concentration of the substance and the thickness of the liquid layer (length of the optical path), and the relationship is as follows:
A=-lg(I/I0)=-lgT=kLc
Where: A is absorbance;
I0 is the incident monochromatic light intensity;
I is the transmitted monochromatic light intensity;
T is the transmittance of the substance; k is the molar absorption coefficient;
L is the optical path of the substance to be analyzed, that is, the side length of the cuvette;
c is the concentration of the substance;
The wavelength of selective absorption of light by a substance, and the corresponding absorption coefficient, is the physical constant of the substance. When the absorption coefficient of a pure substance under certain conditions is known, the test product can be formulated into a solution under the same conditions, and the absorbance can be determined, and the content of the substance in the test sample can be calculated from the above formula. In the visible light region, in addition to the absorption of light by certain substances, many substances are not absorbed by themselves, but can be added to a color developing reagent under certain conditions or processed to make color and then measured, so it is also called colorimetric analysis. Since there are many factors affecting the color depth when color development, and the instrument with poor purity of monochromatic light is often used, the standard or reference material is used for simultaneous measurement.
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