Application and classification of ELISA kits in cellular immunofluorescence - Master's thesis - Dissertation

Before incubating the primary antibody, turn off the incubation time and then do not wash it before adding the primary antibody. Only remove the remaining liquid. After that, do not need to close again, but the primary and secondary antibodies should be diluted with BSA-rich buffer. . Some people also add tween to cut non-specific connections. Closing the antigen can also use the serum of the animal species to which the secondary antibody belongs. The concentration that is not uniformly fixed depends on the condition. Immunofluorescence technique, also known as fluorescent antibody technology, is one of the earliest types of symbolic immunity skills. The first is the ability to use the fluorescent symbol of the antibody to react with the antigen to be tested for the antigen or hapten substance localization. The use of a blocking agent in cellular immunofluorescence Immunofluorescence double-labeling test method and precautions When two antigens are required to be detected together on the same tissue cell specimen, two layers of fluorescent staining are required. The two-layer immunofluorescence labeling method is also divided into a direct method and an indirect method. The process of immunofluorescence staining, several common problems of immunofluorescence skills and the immunofluorescence localization of epidermal growth factor receptor during mouse embryo development. The immunosymbol method can be divided into the following categories: fluorescence immunoassay, radioimmunoassay, Enzyme-linked immunosorbent assay (ELISA), enzyme-coupled biotin-avidin system, time-resolved fluorescence immunoassay, dissociation enhanced lanthanide fluorescence immunoassay. The primary processes of immunofluorescence assay include preparation of cell smears, cell fixation and permeation (or permeabilization), cell closure, primary antibody and secondary antibody incubation, and fluorescence detection.